Publications

Comparison of Co-housing and Littermate Methods for Microbiota Standardization in Mouse Models

Posted by on Jun 3, 2019 in Publications | Comments Off on Comparison of Co-housing and Littermate Methods for Microbiota Standardization in Mouse Models

Robertson SJ, Lemire P, Maughan H, Goethel A, Turpin W, Bedrani L, Guttman DS, Croitoru K, Girardin SE, Philpott DJ Cell Rep. 2019 May 7;27(6):1910-1919.e2. doi: 10.1016/j.celrep.2019.04.023 PMID: 31067473
Abstract: : The intestinal microbiota is a fundamental factor that broadly influences physiology. Thus, studies using transgenic animals should be designed to limit the confounding effects of microbiota variation between strains. Here, we report the impact on intestinal microbiota of co-housed versus F2-generation littermates, two commonly used techniques to standardize...

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Molecular Evolution of Pseudomonas syringae Type III Secreted Effector Proteins

Posted by on Jun 3, 2019 in Publications | Comments Off on Molecular Evolution of Pseudomonas syringae Type III Secreted Effector Proteins

Dillon MM, Almeida RND, Laflamme B, Martel A, Weir BS, Desveaux D, Guttman DS Front Plant Sci. 2019 Apr 5;10:418. doi: 10.3389/fpls.2019.00418 PMID: 31024592
Abstract: Diverse Gram-negative pathogens like Pseudomonas syringae employ type III secreted effector (T3SE) proteins as primary virulence factors that combat host immunity and promote disease. T3SEs can also be recognized by plant hosts and activate an effector triggered immune (ETI) response that shifts the interaction back toward plant immunity. Consequently, T3SEs are pivotal in determining the virulence...

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A Host-Pathogen Interactome Uncovers Phytopathogenic Strategies to Manipulate Plant ABA Responses

Posted by on Jun 3, 2019 in Publications | Comments Off on A Host-Pathogen Interactome Uncovers Phytopathogenic Strategies to Manipulate Plant ABA Responses

Cao FY, Khan M, Taniguchi M, Mirmiran A, Moeder W, Lumba S, Yoshioka K, Desveaux D Plant J. 2019 May 31. doi: 10.1111/tpj.14425 PMID: 31148337
Abstract: The phytopathogen Pseudomonas syringae delivers into host cells type III secreted effectors (T3SEs) that promote virulence. One virulence mechanism employed by T3SEs is to target hormone signalling pathways to perturb hormone homeostasis. The phytohormone abscisic acid (ABA) influences interactions between various phytopathogens and their plant hosts, and has been shown to be a target of P. syringae T3SEs. In order to provide...

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Map of physical interactions between extracellular domains of Arabidopsis leucine-rich repeat receptor kinases.

Posted by on Feb 27, 2019 in Publications | Comments Off on Map of physical interactions between extracellular domains of Arabidopsis leucine-rich repeat receptor kinases.

Mott GA, Smakowska-Luzan E, Pasha A, Parys K, Howton TC, Neuhold J, Lehner A, Grünwald K, Stolt-Bergner P, Provart NJ, Mukhtar MS, Desveaux D, Guttman DS, Belkhadir Y. Sci Data. 2019 Feb 26;6:190025. PMID: 30806640
Abstract: Plants use surface receptors to perceive information about many aspects of their local environment. These receptors physically interact to form both steady state and signalling competent complexes. The signalling events downstream of receptor activation impact both plant developmental and immune responses. Here, we present a comprehensive study of the physical...

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Ca to the rescue – Cachannels and signaling in plant immunity

Posted by on Feb 4, 2019 in Publications | Comments Off on Ca to the rescue – Cachannels and signaling in plant immunity

Moeder W, Phan V, Yoshioka K Plant Sci. 2019 Feb;279:19-26 PMID: 30709488
Abstract: Ca is a universal second messenger in many signaling pathways in all eukaryotes including plants. Transient changes in [Ca]cyt are rapidly generated upon a diverse range of stimuli such as drought, heat, wounding, and biotic stresses (infection by pathogenic and symbiotic microorganisms), as well as developmental cues. It has been known for a while that [Ca]cyt transient signals play crucial roles to activate plant immunity and recently significant progresses have been made in this research field. However the...

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Penicillin binding protein 3 is a common adaptive target among Pseudomonas aeruginosa isolates from adult cystic fibrosis patients treated with β-lactams

Posted by on Jan 23, 2019 in Publications | Comments Off on Penicillin binding protein 3 is a common adaptive target among Pseudomonas aeruginosa isolates from adult cystic fibrosis patients treated with β-lactams

Clark ST, Sinha U, Zhang Y, Wang PW, Donaldson SL, Coburn B, Waters VJ, Yau YCW, Tullis DE, Guttman DS, Hwang DM Int. J. Antimicrob. Agents 2019 Jan; PMID: 30664925
Abstract: Determining the mechanisms that modulate β-lactam resistance in clinical P. aeruginosa isolates can be challenging, as the molecular profiles identified in mutation- or expression-based resistance determinant screens may not correlate with in vitro phenotypes. One of the lesser studied resistance mechanisms in P. aeruginosa is the modification of penicillin binding protein 3 (pbpB/ftsI). Here, we report that...

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A genome-wide association analysis reveals a potential role for recombination in the evolution of antimicrobial resistance in Burkholderia multivorans

Posted by on Jan 21, 2019 in Publications | Comments Off on A genome-wide association analysis reveals a potential role for recombination in the evolution of antimicrobial resistance in Burkholderia multivorans

Diaz Caballero J, Clark ST, Wang PW, Donaldson SL, Coburn B, Tullis DE, Yau YCW, Waters VJ, Hwang DM, Guttman DS PLoS Pathog. 2018 Dec;14(12):e1007453 PMID: 30532201
Abstract: Cystic fibrosis (CF) lung infections caused by members of the Burkholderia cepacia complex, such as Burkholderia multivorans, are associated with high rates of mortality and morbidity. We performed a population genomics study of 111 B. multivorans sputum isolates from one CF patient through three stages of infection including an early incident isolate, deep sampling of a one-year period of chronic infection occurring...

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Identifying Type III Secreted Effector Function via a Yeast Genomic Screen

Posted by on Jan 21, 2019 in Publications | Comments Off on Identifying Type III Secreted Effector Function via a Yeast Genomic Screen

Huei-Yi Lee A, Bastedo DP, Youn JY, Lo T, Middleton MA, Kireeva I, Lee JY, Sharifpoor S, Baryshnikova A, Zhang J, Wang PW, Peisajovich SG, Costanzo M, Andrews BJ, Boone CM, Desveaux D, Guttman DS G3 (Bethesda) 2018 Dec; PMID: 30573466
Abstract: Gram-negative bacterial pathogens inject type III secreted effectors (T3SEs) directly into host cells to promote pathogen fitness by manipulating host cellular processes. Despite their crucial role in promoting virulence, relatively few T3SEs have well-characterized enzymatic activities or host targets. This is in part due to functional redundancy...

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Recombination of ecologically and evolutionarily significant loci maintains genetic cohesion in the Pseudomonas syringae species complex

Posted by on Jan 7, 2019 in Publications | Comments Off on Recombination of ecologically and evolutionarily significant loci maintains genetic cohesion in the Pseudomonas syringae species complex

Dillon MM, Thakur S, Almeida RND, Wang PW, Weir BS, Guttman DS Genome Biol. 2019 Jan;20(1):3 PMID: 30606234
Abstract: BACKGROUND: Pseudomonas syringae is a highly diverse bacterial species complex capable of causing a wide range of serious diseases on numerous agronomically important crops. We examine the evolutionary relationships of 391 agricultural and environmental strains using whole-genome sequencing and evolutionary genomic analyses. RESULTS: We describe the phylogenetic distribution of all 77,728 orthologous gene families in the pan-genome, reconstruct the core genome phylogeny using...

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Arabidopsis ETHYLENE RESPONSE FACTOR 8 (ERF8) has dual functions in ABA signaling and immunity

Posted by on Oct 1, 2018 in Publications | Comments Off on Arabidopsis ETHYLENE RESPONSE FACTOR 8 (ERF8) has dual functions in ABA signaling and immunity

Cao FY, DeFalco TA, Moeder W, Li B, Gong Y, Liu XM, Taniguchi M, Lumba S, Toh S, Shan L, Ellis B, Desveaux D, Yoshioka K BMC Plant Biol. 2018 Sep;18(1):211 PMID: 30261844
Abstract: BACKGROUND: ETHYLENE RESPONSE FACTOR (ERF) 8 is a member of one of the largest transcription factor families in plants, the APETALA2/ETHYLENE RESPONSIVE FACTOR (AP2/ERF) superfamily. Members of this superfamily have been implicated in a wide variety of processes such as development and environmental stress responses. RESULTS: In this study we demonstrated that ERF8 is involved in both ABA and immune signaling. ERF8...

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