Publications

CAGEF has contributed to over 360 publications.

Chloroplast sequence variation and the efficacy of peptide nucleic acids for blocking host amplification in plant microbiome studies

Posted by on Aug 20, 2018 in Publications | Comments Off on Chloroplast sequence variation and the efficacy of peptide nucleic acids for blocking host amplification in plant microbiome studies

Fitzpatrick CR, Lu-Irving P, Copeland J, Guttman DS, Wang PW, Baltrus DA, Dlugosch KM, Johnson MTJ Microbiome 2018 Aug;6(1):144 PMID: 30121081
Abstract: BACKGROUND: The ability to efficiently characterize microbial communities from host individuals can be limited by co-amplification of host organellar sequences (mitochondrial and/or plastid), which share a common ancestor and thus sequence similarity with extant bacterial lineages. One promising approach is the use of sequence-specific peptide nucleic acid (PNA) clamps, which bind to, and block...

read more

Population genomics of bacterial host adaptation

Posted by on Jul 6, 2018 in Publications | Comments Off on Population genomics of bacterial host adaptation

Sheppard SK, Guttman DS, Fitzgerald JR Nat. Rev. Genet. 2018 Jul; PMID: 29973680
Abstract: Some bacteria can transfer to new host species, and this poses a risk to human health. Indeed, an estimated 60% of all human pathogens have originated from other animal species. Similarly, human-to-animal transitions are recognized as a major threat to sustainable livestock production, and emerging pathogens impose an increasing burden on crop yield and global food security. Recent advances in high-throughput sequencing technologies have enabled...

read more

Epidemiology of Clonal Pseudomonas aeruginosa Infection in a Canadian Cystic Fibrosis Population

Posted by on Jun 20, 2018 in Featured Publications, Publications | Comments Off on Epidemiology of Clonal Pseudomonas aeruginosa Infection in a Canadian Cystic Fibrosis Population

Middleton MA, Layeghifard M, Klingel M, Stanojevic S, Yau YCW, Zlosnik JEA, Coriati A, Ratjen FA, Tullis ED, Stephenson A, Wilcox P, Freitag A, Chilvers M, McKinney M, Lavoie A, Wang PW, Guttman DS, Waters VJ Ann Am Thorac Soc 2018 Jun; PMID: 29911888
Abstract: RATIONALE: The extent of the genetic relatedness among Pseudomonas aeruginosa isolates and its impact on clinical outcomes in the cystic fibrosis (CF) population is poorly understood. OBJECTIVES: The objectives of this study were to determine the prevalence of clonal P. aeruginosa...

read more

Structural and biochemical approaches uncover multiple evolutionary trajectories of plant quinate dehydrogenases

Posted by on Jun 12, 2018 in Publications | Comments Off on Structural and biochemical approaches uncover multiple evolutionary trajectories of plant quinate dehydrogenases

Gritsunov A, Peek J, Diaz Caballero J, Guttman D, Christendat D Plant J. 2018 Jun; PMID: 29890023
Abstract: Quinate is produced and used by many plants in the biosynthesis of chlorogenic acids (CGA). CGA are astringent which serves as deterrents of herbivory. They also function as antifungal agents and have potent antioxidant properties. Quinate is produced at a branch point of shikimate biosynthesis by the enzyme, quinate dehydrogenase (QDH). However, little information exists on the identity and biochemical properties of plant QDHs. In this...

read more

Longissimus Dorsi Muscle Transcriptomic Analysis of Yunling and Chinese Simmental Cattle Differing in Intramuscular Fat Content and Fatty Acid Composition

Posted by on Jun 11, 2018 in Publications | Comments Off on Longissimus Dorsi Muscle Transcriptomic Analysis of Yunling and Chinese Simmental Cattle Differing in Intramuscular Fat Content and Fatty Acid Composition

Zhang HM, Xia HL, Jiang HR, Mao YJ, Qu KX, Huang BZ, Gong YC, Yang ZP Genome 2018 Jun; PMID: 29883552
Abstract: Intramuscular fat (IMF) content and fatty acid (FA) composition vary significantly across beef cattle breeds, which play important roles in taste and nutritional value. However, the molecular mechanisms underlying these phenotypic differences remain unknown. The present study compared meat quality traits between Yunling cattle and Chinese Simmental cattle. Yunling cattle showed a lower IMF content and proportion of monounsaturated...

read more

Calmodulin as a Ca2+-Sensing Subunit of Arabidopsis Cyclic Nucleotide-Gated Channel Complexes

Posted by on Feb 21, 2018 in Publications | Comments Off on Calmodulin as a Ca2+-Sensing Subunit of Arabidopsis Cyclic Nucleotide-Gated Channel Complexes

Fischer C, DeFalco TA, Karia P, Snedden WA, Moeder W, Yoshioka K, Dietrich P Plant Cell Physiol. 2017 Jul;58(7):1208-1221 PMID: 28419310
Abstract: Ca2+ serves as a universal second messenger in eukaryotic signaling pathways, and the spatial and temporal patterns of Ca2+ concentration changes are determined by feedback and feed-forward regulation of the involved transport proteins. Cyclic nucleotide-gated channels (CNGCs) are Ca2+-permeable channels that interact with the ubiquitous Ca2+ sensor calmodulin (CaM). CNGCs interact with CaMs via...

read more

Using GCaMP3 to Study Ca2+ Signaling in Nicotiana Species

Posted by on Feb 21, 2018 in Publications | Comments Off on Using GCaMP3 to Study Ca2+ Signaling in Nicotiana Species

DeFalco TA, Toyota M, Phan V, Karia P, Moeder W, Gilroy S, Yoshioka K Plant Cell Physiol. 2017 Jul;58(7):1173-1184 PMID: 28482045
Abstract: Ca2+ signaling is a central component of plant biology; however, direct analysis of in vivo Ca2+ levels is experimentally challenging. In recent years, the use of genetically encoded Ca2+ indicators has revolutionized the study of plant Ca2+ signaling, although such studies have been largely restricted to the model plant Arabidopsis. We have developed stable transgenic Nicotiana benthamiana and Nicotiana...

read more

Diversification of Pseudomonas aeruginosa within the CF lung

Posted by on Feb 6, 2018 in Featured Publications, Publications | Comments Off on Diversification of Pseudomonas aeruginosa within the CF lung

Clark ST, Guttman DS, Hwang DM FEMS Microbiol. Lett. 2018 Feb; PMID: 29401362
Abstract: The evolution and diversification of bacterial pathogens within human hosts represent potential barriers to the diagnosis and treatment of life-threatening infections. Tremendous genetic and phenotypic diversity is characteristic of host adaptation in strains of Pseudomonas aeruginosa that infect the airways of individuals with chronic lung diseases and prove to be extremely difficult to eradicate. In this MiniReview, we examine recent advances in...

read more

Assembly and ecological function of the root microbiome across angiosperm plant species

Posted by on Jan 24, 2018 in Publications | Comments Off on Assembly and ecological function of the root microbiome across angiosperm plant species

Fitzpatrick CR, Copeland J, Wang PW, Guttman DS, Kotanen PM, Johnson MTJ Proc. Natl. Acad. Sci. U.S.A. 2018 Jan; PMID: 29358405
Abstract: Across plants and animals, host-associated microbial communities play fundamental roles in host nutrition, development, and immunity. The factors that shape host-microbiome interactions are poorly understood, yet essential for understanding the evolution and ecology of these symbioses. Plant roots assemble two distinct microbial compartments from surrounding soil: the rhizosphere (microbes surrounding roots)...

read more

An extracellular network of Arabidopsis leucine-rich repeat receptor kinases

Posted by on Jan 19, 2018 in Featured Publications, Publications | Comments Off on An extracellular network of Arabidopsis leucine-rich repeat receptor kinases

Smakowska-Luzan E, Mott GA, Parys K, Stegmann M, Howton TC, Layeghifard M, Neuhold J, Lehner A, Kong J, Grünwald K, Weinberger N, Satbhai SB, Mayer D, Busch W, Madalinski M, Stolt-Bergner P, Provart NJ, Mukhtar MS, Zipfel C, Desveaux D, Guttman DS, Belkhadir Y Nature 2018 01;553(7688):342-346 PMID: 29320478
Abstract: The cells of multicellular organisms receive extracellular signals using surface receptors. The extracellular domains (ECDs) of cell surface receptors function as interaction platforms, and as regulatory modules of receptor...

read more